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fitc anti p2y 1 antibody  (Alomone Labs)


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    Alomone Labs fitc anti p2y 1 antibody
    Fitc Anti P2y 1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p2y+1/Anti-P2Y1+Receptor+-FITC+Antibody/pmc13072548-61-24-28
    Average 94 stars, based on 3 article reviews
    fitc anti p2y 1 antibody - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: Context-Specific Switch from Anti- to Pro-epileptogenic Function of the P2Y 1 Receptor in Experimental Epilepsy
    Article Snippet: Lysis buffer containing phosphatase and protease inhibitors was used to homogenize hippocampal tissue and extract protein, which was quantified using a Tecan plate reader at 560 nm; 30 μg of protein samples was loaded onto an acrylamide gel and separated by SDS-PAGE. .. After the electrophoresis process, proteins were transferred to a nitrocellulose membrane (GE Healthcare) and probed with the following primary antibodies: P2Y 1 (RRID: AB_2040070 ), P2Y 2 (RRID: AB_2040078 ), P2Y 12 (RRID: AB_2040074 ), P2Y 13 (RRID: AB_2040076 , 1/200) (Alomone Labs), GFAP (RRID: AB_477035 , 1/1000) (Sigma-Aldrich), Iba1 (RRID: AB_839504 , 1/400) (Wako), GluR6/7 (RRID: AB_1587072 , 1/1000) (Merck Millipore), synaptophysin (RRID: AB_2286949 , 1/5000) (Abcam), and β-actin (catalog #A5441, 1:1000) (Sigma-Aldrich). .. Membranes were incubated with HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories), and protein bands were visualized using the LAS-4000 system (Fujifilm) with chemiluminescence (Merck Millipore) following analysis using Alpha-EaseFC4.0 software.

    Membrane:

    Article Title: Context-Specific Switch from Anti- to Pro-epileptogenic Function of the P2Y 1 Receptor in Experimental Epilepsy
    Article Snippet: Lysis buffer containing phosphatase and protease inhibitors was used to homogenize hippocampal tissue and extract protein, which was quantified using a Tecan plate reader at 560 nm; 30 μg of protein samples was loaded onto an acrylamide gel and separated by SDS-PAGE. .. After the electrophoresis process, proteins were transferred to a nitrocellulose membrane (GE Healthcare) and probed with the following primary antibodies: P2Y 1 (RRID: AB_2040070 ), P2Y 2 (RRID: AB_2040078 ), P2Y 12 (RRID: AB_2040074 ), P2Y 13 (RRID: AB_2040076 , 1/200) (Alomone Labs), GFAP (RRID: AB_477035 , 1/1000) (Sigma-Aldrich), Iba1 (RRID: AB_839504 , 1/400) (Wako), GluR6/7 (RRID: AB_1587072 , 1/1000) (Merck Millipore), synaptophysin (RRID: AB_2286949 , 1/5000) (Abcam), and β-actin (catalog #A5441, 1:1000) (Sigma-Aldrich). .. Membranes were incubated with HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories), and protein bands were visualized using the LAS-4000 system (Fujifilm) with chemiluminescence (Merck Millipore) following analysis using Alpha-EaseFC4.0 software.

    other:



    Western Blot:

    Article Title: Down-regulation of NTPDase2 and ADP-sensitive P2 Purinoceptors Correlate with Severity of Symptoms during Experimental Autoimmune Encephalomyelitis
    Article Snippet: ecto-5′-nucleotidase/CD73 (rNu-9L(I4,I5)) , Rabbit, pc , 1:100 (FACS) , ectonucleotidases-ab.com;Cat# ecto-5′-nucleotidase/CD73. .. P2Y 1 , Rabbit, pc , 1:1000 (WB) , Alomone Labs; #APR-0009, RRID:AB_2040070. .. P2Y 12 , Rabbit, pc , 1:1000 (WB) , Sigma P4817, RRID:AB_261954.



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    Characterization of platelets from C57BL/6J-P2ry1em1Kcl-Tg(Pf4-icre)Q3Rsko (Plt-P2Y1-/- mice. Tissue taken from pups of PRYC x PRYP parents was used in PCR to determine offspring homozygous for P2Y1- LoxP flanked allele and hemizygous for PF4-cre (test mice) and offspring homozygous for P2Y1- LoxP flanked allele and a non carrier for PF4-cre (control ‘wild type’ mice). Representative PCR for LoxP and cre is shown of a litter ( A ). Platelets were taken via cardiac puncture, or skeletal muscle samples excised and lysed to allow detection of P2Y1R or P2Y12R using western blots using APR-021 antibody ( B ), or APR-009 antibody ( C ). Platelets were also stained with anti-CD41-PE conjugated antibody (platelet marker) and anti-P2Y1-FITC conjugated antibody to elucidate P2Y1R expression between ‘test’ (Plt-P2Y1-/-) and control (WT) platelets ( D ). Data expressed as means +/- SEM. n=3 ( C ). Significant difference represented: *P <0.05, **P <0.01
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    Characterization of platelets from C57BL/6J-P2ry1em1Kcl-Tg(Pf4-icre)Q3Rsko (Plt-P2Y1-/- mice. Tissue taken from pups of PRYC x PRYP parents was used in PCR to determine offspring homozygous for P2Y1- LoxP flanked allele and hemizygous for PF4-cre (test mice) and offspring homozygous for P2Y1- LoxP flanked allele and a non carrier for PF4-cre (control ‘wild type’ mice). Representative PCR for LoxP and cre is shown of a litter ( A ). Platelets were taken via cardiac puncture, or skeletal muscle samples excised and lysed to allow detection of P2Y1R or P2Y12R using western blots using APR-021 antibody ( B ), or APR-009 antibody ( C ). Platelets were also stained with anti-CD41-PE conjugated antibody (platelet marker) and anti-P2Y1-FITC conjugated antibody to elucidate P2Y1R expression between ‘test’ (Plt-P2Y1-/-) and control (WT) platelets ( D ). Data expressed as means +/- SEM. n=3 ( C ). Significant difference represented: *P <0.05, **P <0.01
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    Characterization of platelets from C57BL/6J-P2ry1em1Kcl-Tg(Pf4-icre)Q3Rsko (Plt-P2Y1-/- mice. Tissue taken from pups of PRYC x PRYP parents was used in PCR to determine offspring homozygous for P2Y1- LoxP flanked allele and hemizygous for PF4-cre (test mice) and offspring homozygous for P2Y1- LoxP flanked allele and a non carrier for PF4-cre (control ‘wild type’ mice). Representative PCR for LoxP and cre is shown of a litter ( A ). Platelets were taken via cardiac puncture, or skeletal muscle samples excised and lysed to allow detection of P2Y1R or P2Y12R using western blots using APR-021 antibody ( B ), or APR-009 antibody ( C ). Platelets were also stained with anti-CD41-PE conjugated antibody (platelet marker) and anti-P2Y1-FITC conjugated antibody to elucidate P2Y1R expression between ‘test’ (Plt-P2Y1-/-) and control (WT) platelets ( D ). Data expressed as means +/- SEM. n=3 ( C ). Significant difference represented: *P <0.05, **P <0.01
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    Presence of <t>P2Y</t> 1 and P2Y 12 in dog, cat, and human platelets. ( A , B ) PRP was isolated from the whole blood of dogs, cats, or humans to obtain platelets. Whole lysates of platelets were analysed by immunoblotting using an ( A ) anti-P2Y 1 or ( B ) anti-P2Y 12 receptor antibody (top panels) or ( A , B ) anti-actin antibody (bottom panels). Arrows indicate ( A ) P2Y 1 or ( B ) P2Y 12 receptors. Results are representative of three independent experiments.
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    Image Search Results


    Characterization of platelets from C57BL/6J-P2ry1em1Kcl-Tg(Pf4-icre)Q3Rsko (Plt-P2Y1-/- mice. Tissue taken from pups of PRYC x PRYP parents was used in PCR to determine offspring homozygous for P2Y1- LoxP flanked allele and hemizygous for PF4-cre (test mice) and offspring homozygous for P2Y1- LoxP flanked allele and a non carrier for PF4-cre (control ‘wild type’ mice). Representative PCR for LoxP and cre is shown of a litter ( A ). Platelets were taken via cardiac puncture, or skeletal muscle samples excised and lysed to allow detection of P2Y1R or P2Y12R using western blots using APR-021 antibody ( B ), or APR-009 antibody ( C ). Platelets were also stained with anti-CD41-PE conjugated antibody (platelet marker) and anti-P2Y1-FITC conjugated antibody to elucidate P2Y1R expression between ‘test’ (Plt-P2Y1-/-) and control (WT) platelets ( D ). Data expressed as means +/- SEM. n=3 ( C ). Significant difference represented: *P <0.05, **P <0.01

    Journal: Respiratory Research

    Article Title: Platelet-specific P2Y 1 receptor deficient mice have suppressed pulmonary leukocyte recruitment in response to lipopolysaccharide

    doi: 10.1186/s12931-026-03611-8

    Figure Lengend Snippet: Characterization of platelets from C57BL/6J-P2ry1em1Kcl-Tg(Pf4-icre)Q3Rsko (Plt-P2Y1-/- mice. Tissue taken from pups of PRYC x PRYP parents was used in PCR to determine offspring homozygous for P2Y1- LoxP flanked allele and hemizygous for PF4-cre (test mice) and offspring homozygous for P2Y1- LoxP flanked allele and a non carrier for PF4-cre (control ‘wild type’ mice). Representative PCR for LoxP and cre is shown of a litter ( A ). Platelets were taken via cardiac puncture, or skeletal muscle samples excised and lysed to allow detection of P2Y1R or P2Y12R using western blots using APR-021 antibody ( B ), or APR-009 antibody ( C ). Platelets were also stained with anti-CD41-PE conjugated antibody (platelet marker) and anti-P2Y1-FITC conjugated antibody to elucidate P2Y1R expression between ‘test’ (Plt-P2Y1-/-) and control (WT) platelets ( D ). Data expressed as means +/- SEM. n=3 ( C ). Significant difference represented: *P <0.05, **P <0.01

    Article Snippet: FITC- anti-P2Y 1 antibody (Cat #APR-021-F), anti-P2Y 1 antibody (Cat #APR-021), anti-P2Y 1 antibody (Cat #APR-009) and anti-P2Y 12 antibody (Cat #APR-012) was purchased from Alomone Labs. Stromatol (Cat. #321200S) was purchased from Mascia Brunelli, Italy.

    Techniques: Tissue, Control, Western Blot, Staining, Marker, Expressing, Negative Control, Polymerase Chain Reaction, FACS

    Characterization of platelets from C57BL/6J-P2ry1em1Kcl-Tg(Pf4-icre)Q3Rsko (Plt-P2Y1-/- mice. Tissue taken from pups of PRYC x PRYP parents was used in PCR to determine offspring homozygous for P2Y1- LoxP flanked allele and hemizygous for PF4-cre (test mice) and offspring homozygous for P2Y1- LoxP flanked allele and a non carrier for PF4-cre (control ‘wild type’ mice). Representative PCR for LoxP and cre is shown of a litter ( A ). Platelets were taken via cardiac puncture, or skeletal muscle samples excised and lysed to allow detection of P2Y1R or P2Y12R using western blots using APR-021 antibody ( B ), or APR-009 antibody ( C ). Platelets were also stained with anti-CD41-PE conjugated antibody (platelet marker) and anti-P2Y1-FITC conjugated antibody to elucidate P2Y1R expression between ‘test’ (Plt-P2Y1-/-) and control (WT) platelets ( D ). Data expressed as means +/- SEM. n=3 ( C ). Significant difference represented: *P <0.05, **P <0.01

    Journal: Respiratory Research

    Article Title: Platelet-specific P2Y 1 receptor deficient mice have suppressed pulmonary leukocyte recruitment in response to lipopolysaccharide

    doi: 10.1186/s12931-026-03611-8

    Figure Lengend Snippet: Characterization of platelets from C57BL/6J-P2ry1em1Kcl-Tg(Pf4-icre)Q3Rsko (Plt-P2Y1-/- mice. Tissue taken from pups of PRYC x PRYP parents was used in PCR to determine offspring homozygous for P2Y1- LoxP flanked allele and hemizygous for PF4-cre (test mice) and offspring homozygous for P2Y1- LoxP flanked allele and a non carrier for PF4-cre (control ‘wild type’ mice). Representative PCR for LoxP and cre is shown of a litter ( A ). Platelets were taken via cardiac puncture, or skeletal muscle samples excised and lysed to allow detection of P2Y1R or P2Y12R using western blots using APR-021 antibody ( B ), or APR-009 antibody ( C ). Platelets were also stained with anti-CD41-PE conjugated antibody (platelet marker) and anti-P2Y1-FITC conjugated antibody to elucidate P2Y1R expression between ‘test’ (Plt-P2Y1-/-) and control (WT) platelets ( D ). Data expressed as means +/- SEM. n=3 ( C ). Significant difference represented: *P <0.05, **P <0.01

    Article Snippet: The following primary antibodies were used according to manufacturer’s recommended dilution of 1:200 (concentration of 4 μg/ml): Anti-P2Y 1 R antibody (Alomone Labs, Cat. #APR-021), and Anti-P2Y 12 R Antibody (Alomone Labs, Cat. #APR-012).

    Techniques: Tissue, Control, Western Blot, Staining, Marker, Expressing, Negative Control, Polymerase Chain Reaction, FACS

    Presence of P2Y 1 and P2Y 12 in dog, cat, and human platelets. ( A , B ) PRP was isolated from the whole blood of dogs, cats, or humans to obtain platelets. Whole lysates of platelets were analysed by immunoblotting using an ( A ) anti-P2Y 1 or ( B ) anti-P2Y 12 receptor antibody (top panels) or ( A , B ) anti-actin antibody (bottom panels). Arrows indicate ( A ) P2Y 1 or ( B ) P2Y 12 receptors. Results are representative of three independent experiments.

    Journal: International Journal of Molecular Sciences

    Article Title: P2Y 1 and P2Y 12 Receptors Mediate Aggregation of Dog and Cat Platelets: A Comparison to Human Platelets

    doi: 10.3390/ijms26031206

    Figure Lengend Snippet: Presence of P2Y 1 and P2Y 12 in dog, cat, and human platelets. ( A , B ) PRP was isolated from the whole blood of dogs, cats, or humans to obtain platelets. Whole lysates of platelets were analysed by immunoblotting using an ( A ) anti-P2Y 1 or ( B ) anti-P2Y 12 receptor antibody (top panels) or ( A , B ) anti-actin antibody (bottom panels). Arrows indicate ( A ) P2Y 1 or ( B ) P2Y 12 receptors. Results are representative of three independent experiments.

    Article Snippet: Rabbit anti-human anti-P2Y 1 (cat. No. APR-021) and rabbit anti-human anti-P2Y 12 (cat. No. APR-020) polyclonal antibodies were from Alomone Labs (Jerusalem, Israel).

    Techniques: Isolation, Western Blot